Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Baer J, Boumasmoud M, Shambat SM, Vulin C, Huemer M, Schweizer TA, Gomez-Mejia A, Eberhard N, Achermann Y, Zingg PO, Mestres CA, Brugger SD, Schuepbach RA, Kouyos RD, Hasse B, Zinkernagel AS

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