A quantitative map of human Condensins provides new insights into mitotic chromosome architecture.

Homozygous genome editing was used to fluorescently tag subunits of Condensin I and II in HeLa cells. Absolute abundance, spacing, and dynamic localization of the tagged proteins during mitosis was recorded by fluorescence correlation spectroscopy (FSC)-calibrated live-cell imaging and superresolution microscopy.

Walther N, Hossain MJ, Politi AZ, Koch B, Kueblbeck M, Ødegård-Fougner Ø, Lampe M, Ellenberg J

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