Stepwise-edited, human melanoma models reveal mutations effect on tumor and microenvironment

After euthanizing the mice, solid tumors were collected and fixed with 10% formalin (Patterson Veterinary) for 24 hours. Samples were subsequently transferred into 70% ethanol and submitted to the Histology Core at the Koch Institute for paraffin embedding, in 3 batches, the paraffin blocks were subjected to tissue sectioning, Hematoxylin & Eosin (H&E) staining and digital whole slide scanning.

Hodis E, Torlai Triglia E, Kwon JYH, Biancalani T, Zakka LR, Parkar S, Htter JC, Buffoni L, Delorey TM, Phillips D, Dionne D, Nguyen LT, Schapiro D, Maliga Z, Jacobson CA, Hendel A, Rozenblatt-Rosen O, Mihm Jr MC, Garraway LA, Regev A

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idr0135-hodis-melanocytes/experimentA

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