HIV-1 fusion is disrupted by addition of deoxygluose and is rescued with addition of cholesterol

TZM-bl cells stably expressing CD4 and CCR5 receptors and charged with CCF2, were exposed to HIV-1 pseudoparticles where the Vpr is cloned with BlaM (Vpr-Blam) at MOI = 1. When treating the cells with 100 mM 2-Deoxyglucose (2DG), HIV-1 fusion is blocked. Successive treatment of these cells with 100, 200 and 400 ug/mL of Cholesterol rescues HIV-1 fusion. This experiment shows that cholesterol is key for HIV-1 fusion and that 2DG might downregulate the concentration of cholesterol in the plasma membrane of the host, impeding HIV-1 to effectively fuse in the host. There are 3 different repeats and in each repeat 6 different experiments (images acquired in different conditions).

Coomer CA, Carlon-Andres I, Iliopoulou M, Dustin ML, Compeer EB, Compton AA, Padilla-Parra S

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