Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation

This dataset contains multiview fused images of Tribolium castaneum embryos labelled with a uniform nuclear localized eGFP signal, a uniform Histone-eGFP signal, a uniform LifeAct-eGFP signal, a uniform Tc-Squash-eGFP signal and a Gap43-eYFP signal. Selective Plane Illumination Microscopy (SPIM), was carried out on a Zeiss Lightsheet Z.1 microscope equipped with a 20x/1.0 NA Plan Apochromat water-immersion detection objective and two 10x/0.2 NA dry illumination objectives. Individual views were registered using bead-based registration and then fused to form combined 3D Tiff stacks using the Multiview reconstruction plugin in Fiji.

Jain A, Ulman V, Mukherjee A, Prakash M, Cuenca MB, Pimpale LG, Muenster S, Haase R, Panfilio KA, Jug F, Grill SW, Tomancak P, Pavlopoulos A

Browse Data in IDR

idr0099-jain-beetlelightsheet/experimentA ()

BioFile Finder

BioFile Finder is a tool for filtering, sorting and grouping tabular data. Each table below is loaded from IDR as a Parquet file, with each row representing an image. Images can be selected in BioFile Finder and the "Download" link will open them in the IDR viewer.

NB: large tables may take some time to load in BioFile Finder.

experimentA ( , parquet: -)

Download

To download original image files in your browser, you can access original data.

Download as JSON.

For more download options, including bulk download using Globus or FTP clients, see the IDR Data download page.