Tales of 1,008 Small Molecules: Phenomic Profiling through Live-cell Imaging in a Panel of Reporter Cell Lines

We screened a library of 1,008 approved drugs and well-characterized tool compounds manually annotated to 218 unique mechanisms-of-action (MoA) and 169 natural products at four concentrations with four replicates each in live-cell, high-content imaging screens against a panel of 15 reporter cell lines, which expressed a diverse set of fluorescent organelle and pathway markers in three distinct cell lineages. Each reporter cell line carried a BFP segmentation cassette that enabled detection of the nuclear and cell boundaries. In addition, each reporter cell line expressed a GFP- and a RFP/FusionRed-tagged organelle or pathway marker from its endogenous chromosomal locus. These markers were selected to cover a broad array of organelles, cellular structures and regulatory pathways for the generation of information-rich image-based compound descriptors.

Cox MJ, Jaensch S, Van de Waeter J, Cougnaud L, Seynaeve D, Benalla S, Koo SJ, Van Den Wyngaert I, Neefs JM, Malkov D, Bittremieux M, Steemans M, Peeters PJ, Wegner JK, Ceulemans H, Gustin E, Chong YT, Gšhlmann HWH

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