Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy

We have adapted the mouse kidney rudiment assay to generate renal organoids. We have immunostained endpoint fixed organoids and E13.5 embryonic kidneys and imaged them with a light sheet fluorescent microscope. The data generated for this manuscript is made accessible here and includes immunofluorescence staining after end point fixation revealing that organotypic structures develop within the organoids. Furthermore, we have performed an assay to ascertain tubular functionality. Following, we have imaged organoids containing Wt1-GFP knock-in cells live on the light sheet microscope to evaluate the ability to follow cells long-term and generate previously unavailable quantitative tracking data.\n\nidr0038 consists of a set of 3 experiments: experimentA (immunofluorescence staining after end point fixation), experimentB (assays to ascertain tubular functionality) and experimentC (Wt1-GFP knock-in cells followed long term).

Held M, Santeramo I, Wilm B, Murray P, Lévy R

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idr0038-held-kidneylightsheet/experimentB ()

idr0038-held-kidneylightsheet/experimentC ()

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