Human U2OS cells - compound-profiling Cell Painting experiment

The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the "Cell Painting" assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format. The plates in this screen were originally included as part of idr0016/screenA but were moved to idr0036/screenA in August 2017 to make it clearer that they correspond to the Broad BioImaging Benchmark Collection dataset BBBC022 (https://www.broadinstitute.org/bbbc/BBBC022/). Version History The plates in this screen were originally included as part of idr0016/screenA but were moved to idr0036/screenA in August 2017 to make it clearer that they correspond to the Broad BioImaging Benchmark Collection BBBC022 (https://www.broadinstitute.org/bbbc/BBBC022/).

Gustafsdottir SM, Ljosa V, Sokolnicki KL, Anthony Wilson J, Walpita D, Kemp MM, Petri Seiler K, Carrel HA, Golub TR, Schreiber SL, Clemons PA, Carpenter AE, Shamji AF

Browse Data in IDR

idr0036-gustafsdottir-cellpainting/screenA ()

BioFile Finder

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screenA ( , parquet: -)

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View OME-Zarr data and download at BioImage Archive: S-BIAD855.

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