RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer.

In order to metastasize triple negative breast cancer (TNBC) must make dynamic changes in cell shape. The shape of all eukaryotic cells is regulated by Rho GTP Exchange Factors (RhoGEFs), which activate Rho-family GTPases in response to mechanical and informational cues. In contrast, Rho GTPase-activating proteins (RhoGAPs) inhibit Rho GTPases. However, which RhoGEFs and RhoGAPS couple TNBC cell shape to changes in their environment is very poorly understood. Moreover, whether the activity of particular RhoGEFs and RhoGAPs become dysregulated as cells evolve the ability to metastasise is not clear. Towards the ultimate goal of identifying RhoGEFs and RhoGAPs that are essential for TNBC metastasis, we performed an RNAi screen to isolate RhoGEFs and RhoGAPs that contribute to the morphogenesis of the highly metastatic TNBC cell line LM2, and its less-metastatic parental cell line MDA-MB-231. For ~6 million cells from each cell line we measured 127 different features following the depletion of 142 genes. Using a linear classifier scheme we also describe the morphological heterogeneity of each gene-depleted population. Version History July 2017: Replaced cell line name of MDA-MB-231 with MDAMB231 in screens C and D to be inline with EFO cell line name.

Pascual-Vargas P, Cooper S, Sero J, Bousgouni V, Arias-Garcia M, Bakal C

Browse Data in IDR

idr0028-pascualvargas-rhogtpases ()

idr0028-pascualvargas-rhogtpases/screenA ()

idr0028-pascualvargas-rhogtpases/screenB ()

idr0028-pascualvargas-rhogtpases/screenC ()

idr0028-pascualvargas-rhogtpases/screenD ()

BioFile Finder

BioFile Finder is a tool for filtering, sorting and grouping tabular data. Each table below is loaded from IDR as a Parquet file, with each row representing an image. Images can be selected in BioFile Finder and the "Download" link will open them in the IDR viewer.

NB: large tables may take some time to load in BioFile Finder.

idr0028-pascualvargas-rhogtpases ( , parquet: -)

screenA ( , parquet: -)

screenB ( , parquet: -)

screenC ( , parquet: -)

screenD ( , parquet: -)

Download

To download original image files in your browser, you can access original data.

Download as JSON.

For more download options, including bulk download using Globus or FTP clients, see the IDR Data download page.