High resolution imaging reveals heterogeneity in chromatin states between cells that is not inherited through cell division.

To monitor the organisation of chromatin in live cells were engineered with fluorescent fusion proteins which recognize specific operator sequences to tag pairs of syntenic gene loci. The separation of these loci was then tracked in three dimensions over time using fluorescence microscopy. Data generated for this manuscript are made accessible here. This includes the movies generated from yeast strains with different genomic separations. These are available within subfolders titled "Colocalising", "Genomic separation 100kb", "Genomic separation 25kb" etc. Tracking data generated from these movies is also available as idr0027-AnalysisAllData.csv in the Attachments section below. This includes x, y and z co-ordinates of foci in the red and green channels following two stages of correction for channel alignment, the distance between the red and green foci in nanometers, the offset of the red channel foci with respect to the green channel foci in x, y, and z. The maximum intensity, background intensity and standard deviation of the background intensity for both the red and green channels. The contrast (maximum spot intensity - nuclear background intensity) for each channel. The name originally assigned to the video, the genomic separation of the strain in kb. The string in column AE starts with the letter assigned to the video in supplementary figure 2. Some videos include two cells. In these cases the higher cell (on the y axis) is indicated as "upper" and the other as "lower". One of the two cells can often be assigned as the mother and the other as daughter. This is indicated as m or d in column AF. The systematic name of the strain is indicated in column AG.

Dickerson D, Gierliński M, Singh V, Kitamura E, Ball G, Tanaka TU, Owen-Hughes T

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