Human U2OS cells - compound cell-painting experiment

Using the cell-painting assay developed by Gustafsdottir et al, 2013, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The experiment consisted of 413 microtiter plates. Each plate has 384 wells. Each well has 6 fields of view; a very small fraction of the wells (0.002%) have a few sites missing. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file. Version History In August 2017 the 20 plates from the Broad Bioimaging Benchmark Collection dataset BBBC022 were moved to a separate screen, idr0036/screenA. At the same time 58 new plates, which had become available from the Cell Image Library (http://www.cellimagelibrary.org/pages/project_20269) after the original import to idr0016, were added to idr0016 and annotation was added to plate 25723 (https://idr.openmicroscopy.org/webclient/?show=plate-5104) which was previously unannotated. Protocols have been updated to reflect the materials and methods information in Wawer et al 2014.

Wawer MJ, Li K, Gustafsdottir SM, Ljosa V, Bodycombe NE, Marton MA, Sokolnicki KL, Bray MA, Kemp MM, Winchester E, Taylor B, Grant GB, Hon CS, Duvall JR, Wilson JA, Bittker JA, Dančík V, Narayan R, Subramanian A, Winckler W, Golub TR, Carpenter AE, Shamji AF, Schreiber SL, Clemons PA,Bray MA, Gustafsdottir SM, Rohban MH, Singh S, Ljosa V, Sokolnicki KL, Bittker JA, Bodycombe NE, Dancík V, Hasaka TP, Hon CS, Kemp MM, Li K, Walpita D, Wawer MJ, Golub TR, Schreiber SL, Clemons PA, Shamji AF, Carpenter AE

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idr0016-wawer-bioactivecompoundprofiling/screenA ()

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screenA ( , parquet: -)

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