Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH

Datasets 1 and 2 are replicates of the same experimental design which consists of 16 probes targeting 16 consecutive loci on chromosome 2 using iFISH4U. The 16 probes targeting different loci are spaced with 3 or 20 Mb in order to study relatively small genomic distances and compare them with larger distances. Furthermore, 10 loci are made by a combination of two colours alternating within the locus and 6 loci have only one colour. Dataset 3 is a single colour experiment where one probe with alternated colours (AF488-AF594) was tested together with other two probes in different colours located in distinct genomic sites. There is 1 probe with AF488 dye, 1 probe with AT542, 1 probe with AF594 and 1 probe with AT647N. Dataset 4 is a single colour experiment where one probe with alternated colours (AT542-AT647N) was tested together with two other probes in different colours located in distinct genomic sites. There is 1 probe with AF488 dye, 1 probe with AT542, 1 probe with AF594 and 1 probe with AT647N. Dataset 5 consists of 23 probes targeting 23 consecutive loci on chromosome 1 using iFISH4U. The 23 loci are spaced with 10 Mb. There are 3 probes with AF488 dye, 4 probes with AT542, 4 probes with AF594, 4 probes with AT647N, 4 probes with AF700 and 4 probes with AF790. Datasets 6 and 7 are replicates of the same experimental design which consists of 21 probes targeting 21 consecutive loci on chromosome 2 using iFISH4U. The 23 loci are spaced with 10 Mb. There are 3 probes with AF488 dye, 3 probes with AT542, 3 probes with AF594, 3 probes with AT647N, 6 probes with AF700 and 3 probes with AF790. Datasets 8 and 9 are replicates of the same experimental design which consists of 13 probes targeting 13 consecutive loci on chromosome 10 using iFISH4U. The 23 loci are spaced with 10 Mb. There are 2 probes with AF488 dye, 2 probes with AT542, 3 probes with AF594, 2 probes with AT647N, 2 probes with AF700 and 2 probes with AF790.

Mota A, Schweitzer M, Wernersson E, Crosetto N, Bienko M

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